Technical Data
029447
CNPase (2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP) (FITC)
Description:
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by this monoclonal antibody. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
U251 cells

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG113A169Affinity Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified human brain CNPase.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human CNPase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sung-Min A., et al. (2008). PLoS ONE. 3:e3917. 2. Goetz A., et al. (2006). Proc. Natl. Acad. Sci. U.S.A. 103: 11063-8. 3. Jason D., et al. (2006). The Journal of comparative neurology. 495:351-62.