Technical Data
029451
Dab2, CT (Disabled Homolog 2, DOC-2, Differentially-expressed Protein 2, DOC2)
Description:
Disabled-2 is a component of the CSF-1 signal transduction pathway. Dab2 is involved in the regulation of cytoskeleton based functions by regulating cell positioning, inducing marcrophage adhesion, or regulation of protein trafficking of endocytosis involved proteins. Dab2 has been implicated in regulating the homeostasis of epithelial differentiation, where Dab2 is involved in endodermal cell formation, differentiation, and organization. Dab2 mRNA expression is common in normal ovarian epithelia cells but low or lack of expression is seen in ovarian carcinoma cell lines.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:20
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Human pancreas tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13A171Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Dab2 at C-terminal.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Dab2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morris, S.M., et al. (2002). EMBO J. 21:1555-1564. 2. Sheng, Z., et al. (2000). Oncogene. 19:4847-4854. 3. Mok, S.C., et al. (1994). Gynecol. Oncol. 52:247-252.