		Technical Data
029462	ENDOG (Endonuclease G, Mitochondrial, Endo G)

Description:
Endonuclease G is a vertebrate nuclease that is found in the mitochondria and is thought to be important in mitochondrial DNA (mtDNA) replication. Endo G has also been found to have apoptosis-inducing behavior. Endo G apoptosis is thought to be induced by benzyl isothiocyanate.

Applications:
Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
NIH/3T3 cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic linear peptide corresponding to human Endonuclease G (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human Endonuclease G. Species Crossreactivity: mouse. Species sequence homology: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Liu, K.C., et al. (2011). Int J Oncol. 38(3):787-796. 2. Chiu, T.H., et al. (2009). Anticancer Res. 29(11):4503-4511. 3. Prats, E., et al. (1997). DNA Cell Biol. 16(9): 1111-1122.