Technical Data
029462
ENDOG (Endonuclease G, Mitochondrial, Endo G)
Description:
Endonuclease G is a vertebrate nuclease that is found in the mitochondria and is thought to be important in mitochondrial DNA (mtDNA) replication. Endo G has also been found to have apoptosis-inducing behavior. Endo G apoptosis is thought to be induced by benzyl isothiocyanate.

Applications:
Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
NIH/3T3 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human Endonuclease G (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Endonuclease G. Species Crossreactivity: mouse. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, K.C., et al. (2011). Int J Oncol. 38(3):787-796. 2. Chiu, T.H., et al. (2009). Anticancer Res. 29(11):4503-4511. 3. Prats, E., et al. (1997). DNA Cell Biol. 16(9): 1111-1122.