Technical Data
029490
H2AFB1 (Histone H2A-Bbd Type 1, H2A Barr Body-deficient, H2A.Bbd)
Description:
Histone H2A.Bbd also known as histone H2A-Bbd type 1, is an atypical histone variant. This histone variant associates with H2B and replaces histone H2A in the nucleosome. In contrast to other H2A histones, this variant lacks conserved residues that are targets of post-translational modifications. Consequently this H2A.Bbd variant is about 15aa shorter and only 48% similar to the canonical H2A. The major differences between the two are found in the docking domain that links H2A -H2B to the H3-H4 tetramer. Nucleosomes containing this histone adopt a more relaxed structure and protect ~118 base pairs of DNA instead of the 147 base pairs found in classical nucleosomes. This variant histone is associated with active X chromosomes and autosomes, and is typically absent from inactive X chromosomes.

Applications:
Suitable for use in Western Blot and Chromatin Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
Normal rabbit IgG and primers specific for human PGK1.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
25Tests-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic linear peptide corresponding to human Histone H2A, Bbd (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4,150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Histone H2A.Bbd. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.