Technical Data
029494
HELLS (Lymphoid-specific Helicase, Proliferation-associated SNF2-like Protein, SWI/SNF2-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 6, PASG, SMARCA6, Nbla10143)
Description:
A member of the SNF2 family of chromatin remodeling proteins, Lymphoid-specific helicase (HELLS, also known as Lsh) assumes various roles in normal development, survival, de novo or maintenance DNA methylation, gene silencing, and heterochromatin formation and organization. It is shown to serve as cancer progression marker in conjunction with CEP55 and FOXM1 for the detection of head and neck squamous cell carcinoma. It is also reported that expression of Lsh is crucial for meiotic progression in mouse spermatocytes. Lsh is shown to directly associate with the 5' DMR of the Cdkn1c promoter, and thereby regulate its gene expression. Additionally, Lsh is reported as a regulator of repressive chromatin at retrotransposons, and is demonstrated to mediate the silencing of stem cell-specific genes such as Oct4.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 2ug/ml
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa nuclear extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to he coiled-coil region of human Lymphoid-specific helicase (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human HELLS. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huang, J., et al. (2004). Nucleic Acids Res. 32(17):5019-5028. 2. Fan, T., et al. (2005). Development. 132(4): 635-644. 3. Zhu, H., et al. (2006). EMBO J. 25(2):335-345. 4. Waseem, A., et al. (2010). Oral Oncol. 46(7): 536-542. 5. Zeng, W., et al. (2011). Biol Reprod. 84(6): 1235-1241. 6. Xi, S., et al. (2009). Stem Cells. 27(11):2691-2702.