		Technical Data
029507	HOXD4 (Homeobox Protein Hox-D4, Homeobox Protein HHO.C13, Homeobox Protein Hox-4B, Homeobox Protein Hox-5.1, HOX4B)

Description:
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. It is reported that all Hox paralog group 4 members, A4, B4, C4, and D4, have similar effects on hematopoietic stem and progenitor self-renewal in vitro. It is shown that each paralog group 4 member both promotes proliferation and inhibits differentiation, thereby enabling the exponential expansion of hematopoietic progenitors from c-kit(+)/CD41(+) cell fractions of day 6 murine embryoid bodies.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat kidney tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM,k	13A198	Highly Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Mouse

Concentration:

As reported

Immunogen:

Recombinant corresponding to human HOXD4 fused to GST-tag.

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes HOXD4. Species Crossreactivity: mouse and rat. Species sequence homology: human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Phelan, M.L., Sadoul, R., Featherstone, M.S. (1994). Mol Cell Biol. 14(8):5066-5075. 2. Lacovino, M., et al. (2009). Stem Cells Dev. 18(5):783-792. 3. Gregoire, D., Brodolin, K., and Mechali, M. (2006). EMBO Rep. 7(8):812-816. 4. Ferretti, E., et al. (2005). Mol Cell Biol. 25(19):8541-8542.