Technical Data
IGF2BP3 (Insulin-like Growth Factor 2 mRNA-binding Protein 3, IGF2 mRNA-binding Protein 3, IMP-3, IGF-II mRNA-binding Protein 3, KH Domain-containing Protein Overexpressed in Cancer, hKOC, VICKZ Family Member 3, IMP3, KOC1, VICKZ3)
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor 2 leader 3 mRNA and may repress translation of insulin-like growth factor 2 during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

Suitable for use in Western Blot, Immunoprecipitation and RNA Binding Protein Immunoprecipitation assay. Other applications not tested.

Recommended Dilution:
RIP: ~5ug/RIP
Optimal dilutions to be determined by the researcher.

Positive Control:
Includes negative control normal rabbit IgG antibody and control primers specific for the cDNA of human IGF2.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
10Tests-20CBlue IceHumanRabbit
Synthetic linear peptide corresponding to human IGF2 at C-terminal (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide, 30% glycerol.
Recognizes human IGF2. Species Crossreactivity: mouse. Species sequence homology: Chimpanzee, rhesus monkey, canine, equine (100%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nielsen,J.,etal.(1999).Molec.Cell.Biol.19: 1262-1270. 2. Martens, J.W., et al. (2003). Thromb Haemost. 89(2):393-404.