Technical Data
029512
IGF2BP3 (Insulin-like Growth Factor 2 mRNA-binding Protein 3, IGF2 mRNA-binding Protein 3, IMP-3, IGF-II mRNA-binding Protein 3, KH Domain-containing Protein Overexpressed in Cancer, hKOC, VICKZ Family Member 3, IMP3, KOC1, VICKZ3)
Description:
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor 2 leader 3 mRNA and may repress translation of insulin-like growth factor 2 during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and RNA Binding Protein Immunoprecipitation assay. Other applications not tested.

Recommended Dilution:
RIP: ~5ug/RIP
Optimal dilutions to be determined by the researcher.

Positive Control:
Includes negative control normal rabbit IgG antibody and control primers specific for the cDNA of human IGF2.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
10Tests-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic linear peptide corresponding to human IGF2 at C-terminal (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 30% glycerol.
Specificity:
Recognizes human IGF2. Species Crossreactivity: mouse. Species sequence homology: Chimpanzee, rhesus monkey, canine, equine (100%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nielsen,J.,etal.(1999).Molec.Cell.Biol.19: 1262-1270. 2. Martens, J.W., et al. (2003). Thromb Haemost. 89(2):393-404.