Technical Data
KEAP1 (Kelch-like ECH-associated Protein 1, Cytosolic Inhibitor of Nrf2, INrf2, Kelch-like Protein 19, INRF2, KIAA0132, KLHL19)
Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.

Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunocytochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic linear peptide corresponding to human KEAP1 (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Recognizes human KEAP1. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lee, S., et al. (2011). World J Cardiol. 3(1):18- 24. 2. Kundu, J.K., et al. (2010). Pharm Res. 27(6): 999-1013. 3. Li, C.Q., et al. (2009). Proc Natl Acad Sci USA. 106(34):14547-14551.