Technical Data
029551
NEFH (Neurofilament Heavy Polypeptide, NF-H, 200kD Neurofilament Protein, Neurofilament Triplet H Protein, KIAA0845, NFH)
Description:
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments 10nm in dia. are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins 68/70, 160, and 200kD occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Bovine cerebellum tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to bovine Neurofilament H fused to GST-tag.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes bovine Neurofilament H. Species sequence homology: Human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Karlsson, J., et al. (1989). J Neurochem. 53(3): 759-765. 2. Clark, B., et al. (2003). J Neurotrama. 20(7): 681-695. 3. Neunlist, M., et al. (2011). Gastrointest Ensosc. 73(5):1029-1034. 4. Colakoglu, G. and Brown, A. (2009). J Cell Biol. 185(5): 769-777. 5. Alami, N.H., et al. (2009). J Neurosci. 29(20): 6625-6634. 6. Kudryshova, E., et al. (2009). Hum Mol Genet. 18(7):1353-1367. 7. Yanping, Y., Jensen, K., and Brown, A., (2007). Cell Motil Cytoskeleton. 65(4):299-309. 8. Hanz, S., et al. (2003). Neuron. 40(6):1095-1104. 9. Brazelton, T.R., et al. (2000). Science. 290(5497):1776-1179. 10. Cheung, W.M., et al. (1999). Biotechniques. 26(5):946-948. 11. Benuck, M., et al. (1996). J Neurochem. 67(5):2019-2029. 12. Harris, J., Ayyub, C., and Shaw, G. (1991). J Neurosci Res. 30(1):47-62. 13. Wallquist, W., et al. (2005). J Neuroscience. 25(14):3692-3700.