Technical Data
029579
Pericentrin (Kendrin, Pericentrin-B, PCNT, KIAA0402, PCNT2)
Description:
Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human Pericentrin (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Pericentrin. Species sequence homology: Mouse (89%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Delaval, B., et al. (2010). J Cell Biol. 188(2): 181-190. 2. Griffith E., et al. (2008). Nat Genet. 40(2): 232- 236. 3. Rauch, A., et al. (2008). Science. 319(5864): 816-819. 4. Pujana, M.A., et al. (2007). Nat Genet. 39(11): 1338-1349. 5. Wen, Y., et al. (2008). J Biol Chem. 283(5): 2793-2803.