Technical Data
029646
ABO, Recombinant, Human, aa54-354, His-Tag (Histo-blood Group ABO System Transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Histo-blood Group A Transferase, Histo-blood Group B Transferase, NAGAT)
50ug
250ug
Molecular Biology Storage: -20CShipping: Blue Ice
ABO, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.

Source:
Recombinant corresponding to aa54-354 from human ABO, fused to His-tag at N-terminal expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP

Molecular Weight:
~37.4kD (MALDI-TOF)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~37.4kD
Source: E.coli
Purity: ~85% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 20% glycerol, 200mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Persson M., et al. (2007) J. Biol. Chem. 282:9564-9570. 2. Nydegger, U.E., et al. (2005) Ann. N.Y. Acad. Sci. 1050: 40-51.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.