Technical Data
029723
ATG10 (Ubiquitin-like-conjugating Enzyme ATG10, Autophagy-related Protein 10, APG10-like, APG10L, PP12616)
Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomiclysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 (Also called ATG10, APG10L), an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12- ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to human APG10 at C-terminus.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.5mg/ml BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human APG10. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.