Technical Data
029802
IFNG (Interferon gamma, IFN-gamma, Immune Interferon)
Description:
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigenspecific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Murine IFN-gamma is a 15.6kD protein containing 134aa residues.

Applications:
Suitable for use in Western Blot, ELISA and Neutralization. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
ELISA: 2-3ug/ml
Neutralization: 0.2-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to mouse IFN-gamma expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes mouse IFN-gamma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.