Technical Data
029814
IL17A (Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated Antigen 8, CTLA-8, CTLA8, IL17)
Description:
IL-17 was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. The cytokine exhibits a high degree of aa identity with HVS13, an open reading frame from a T lymphotropic Herpes virus saimiri and with murine CTLA8. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. IL-17 is a disulfide-linked homodimer of two 31kD subunits, each containing 136aa residues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 2-4ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to human IL-17 expressed in E.coli.
Purity:
Purified by affinity chromatography employing immobilized human IL-17 matrix.
Form
Supplied as a liquid in PBS, pH 7.2, 10mM EDTA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human IL-17.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.