		Technical Data
029814	IL17A (Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated Antigen 8, CTLA-8, CTLA8, IL17)

Description:
IL-17 was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. The cytokine exhibits a high degree of aa identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri and with murine CTLA8. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. IL-17 is a disulfide-linked homodimer of two 31kD subunits, each containing 136aa residues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 2-4ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Recombinant protein corresponding to human IL-17 expressed in E.coli.

Purity:

Purified by affinity chromatography employing immobilized human IL-17 matrix.

Form

Supplied as a liquid in PBS, pH 7.2, 10mM EDTA, 0.02% thimerosal, 50% glycerol.

Specificity:

Recognizes human IL-17.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.