Technical Data
Insig1 (Insulin-induced Gene 1 Protein, INSIG-1, Immediate-early Protein CL-6, Insulin-induced Growth Response Protein CL-6, Cl-6)
Insulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRatRabbit
Synthetic peptide surrounding aa248 from rat Insig1.
Purified by affinity chromatography.
Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Recognizes rat InSig-1 at ~30kD and its precursor (~50kD). Species Crossreactivity: human, mouse, hamster and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.