Technical Data
029929
ABCG2 (ATP-binding cassette sub-family G member 2)
Description:
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Applications:
Western Bloting: 1:500-1:2000.
Immunohistochemistry: 1:200-1:1000.
Immunofluorescence: 1:200-1:1000.
Flow cytometry: 1:200-1:400.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B7
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human ABCG2 expressed in E. coli.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human, Mouse, Monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carcinogenesis. 2008 Dec;29(12):2289-97.
2. Pharm Res. 2009 Feb;26(2):449-58.