Technical Data
029938
Fetoprotein, Alpha (Alpha Fetoprotein, Alpha, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP)
Description:
AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b13B16
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human AFP expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7160-4.
2. Pediatr Hematol Oncol. 2001 Dec;18(8):509-18.
3. Tumour Biol. 2002 Jul-Aug;23(4):202-11.