Technical Data
029960
APOA5, Antibody Set, BioAssay™
100ul
Kits and Assays Storage: -20°CShipping: Blue Ice
Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50–70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor- agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum

This antibody pair detected recombinant apoa5 protein in sandwich ELISA format and could be potential reagents for the development of clinical diagnostic kits.

Applications:
Western Bloting: 1:500-1:2000.
Immunofluorescence: 1:200-1:1000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity: Purified
Concentration: Not determined
Form: Purified antibody in PBS, 0.03% sodium azide.
Specificity: Human

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1 Pennacchio, L Science 2001. 294, 169-173.
2 Prieur, X. J Biol Chem 2003. 278, 25468-25480.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.