Technical Data
029993
BLK (B lymphoid tyrosine kinase)
Description:
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .

Applications:
Western Bloting: 1:500-1:2000.
Immunofluorescence: 1:200-1:1000.
Flow cytometry: 1:200-1:400.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B71
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human BLK expressed in E. coli.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. N Engl J Med. 2008 Feb 28;358(9):900-9.
2. Genes Immun. 2009 Apr;10(3):219-26.
3. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14460-5.