Technical Data
030007
BTK (Tyrosine-protein Kinase BTK, Agammaglobulinaemia Tyrosine Kinase, ATK, B Cell Progenitor Kinase, BPK, Bruton Tyrosine Kinase, AGMX1, ATK, BPK)
Description:
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Applications:
Western Bloting: 1:500-1:2000.
Immunohistochemistry: 1:200-1:1000.
Immunofluorescence: 1:200-1:1000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B85
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of BTK expressed in E. coli.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human, Monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240.
2. Thomas, J.D., et al. 1993. Science. 261: 355-358.
3. Tamagnone, L., et al. Oncogene 9: 3683-3688.