Technical Data
COX4I1 (Cytochrome c oxidase subunit IV isoform 1)
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.

Western Bloting: 1:500-1:2000.
Immunofluorescence: 1:200-1:1000.
Flow cytometry: 1:200-1:400.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanMouse
Not determined
Purified recombinant fragment of human COX4I1 expressed in E. coli.
Ascites fluid, 0.03% sodium azide.
Human, Monkey, Rat, Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Biochim Biophys Acta. 1992 Feb 26;1119(2):218-24.
2. Histochemistry. 1990;94(2):211-5.
3. FEBS Lett. 2000 Jun 30;476(1-2):22-6.