Technical Data
030122
CSK (C-Src kinase)
Description:
Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.

Applications:
Western Bloting: 1:500-1:2000.
Immunofluorescence: 1:200-1:1000.
Flow cytometry: 1:200-1:400.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B200
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human CSK expressed in E. coli.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human, Mouse, Monkey, Rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nat Genet. 2009 Jun;41(6):677-87.
2. Leuk Res. 2009 Sep;33(9):e168-9.
3. J Hypertens. 2011 Jan;29(1):62-9.