Technical Data
DDR2 (discoidin domain receptor family, member 2) is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/ threonine (STK) kinase catalytic domains. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.

Western Bloting: 1:500-1:2000.
Immunohistochemistry: 1:200-1:1000.
Immunofluorescence: 1:200-1:1000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanMouse
Not determined
Purified recombinant fragment of human DDR2 expressed in E. coli.
Ascites fluid, 0.03% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leitinger B. Kwan AP. Matrix Biol. 2006, Aug, 25(6):355-64. Epub 2006 May 26.
2. Shyu KG. Chao YM. Wang BW. et al. Hypertension. 2005, Sep, 46(3):614-21. Epub 2005 Aug 8.
3. Neale JC. Kenny TP. Gershwin ME. Clin Dev Immunol. 2004, Jun, 11(2):157-63.