Technical Data
030149
DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)
Description:
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50-55aa in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12aa. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Applications:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Flow Cytometry: 1:200-1:400
ELISA: 1:10000

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B227
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. coli.
Purity:
Purified
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Exp Dermatol. 2011 Mar;20(3):273-7. 2.Thromb Haemost. 2011 Jan;105(1):72-80.