Technical Data
030281
Glutathione S-Transferase (GST)
Description:
Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B359
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Purified fusion protein with GST protein tag expressed in E. coli.
Purity:
Purified
Form
Purified antibody in PBS, 0.03% sodium azide.
Specificity:
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. 1993. Biotechnology (N Y) 11: 933-6.
2. 1988. Gene 67: 31-40.