Technical Data
030323
IgG, Human
Description:
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B401
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human IgG was isolated from human sera and purified by chromatography.
Purity:
Purified
Form
Purified antibody in PBS, 0.03% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
2. Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).