Technical Data
030329
IKBKE
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Technical Data |
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030329 |
IKBKE |
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Description: Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NF B is retained in the cytoplasm in an inactive form by the inhibitory protein I B. Activation of NF B requires that I B be phosphorylated on specific serine residues, which results in targeted degra-dation of I B. I B kinase (IKK ), previously designated CHUK, interacts with I B- and specifically phosphorylates I B- on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKK , IKK and IKK (also designated NEMO), and each appear to make essential contributions to I B phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKK and IKK . IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNF , IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of I B on Ser32 and Ser36, and in NF B activation, suggesting that IKK-i may act as an I B kinase in the immune system. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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