Technical Data
030329
IKBKE
Description:
Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NF B is retained in the cytoplasm in an inactive form by the inhibitory protein I B. Activation of NF B requires that I B be phosphorylated on specific serine residues, which results in targeted degra-dation of I B. I B kinase (IKK ), previously designated CHUK, interacts with I B- and specifically phosphorylates I B- on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKK , IKK and IKK (also designated NEMO), and each appear to make essential contributions to I B phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKK and IKK . IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNF , IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of I B on Ser32 and Ser36, and in NF B activation, suggesting that IKK-i may act as an I B kinase in the immune system.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B407
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of IKBKE (aa1-257) expressed in E. coli.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cell. 2007 Jun 15;129(6):1065-79.
2. Mol Syst Biol. 2007;3:89. Epub 2007 Mar 13.
3. Arthritis Rheum. 2007 Mar;56(3):743-52.