		Technical Data
030335	IL-6

Description:
Interleukin 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Igsecreting cells, and induces myeloma, plasmacytoma growth, nerve cells differentiations. In addition, IL-6 is an important regulator of acute phase reaction.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	13B413

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Purified recombinant fragment of human IL-6 expressed in E. coli.

Purity:

Ascites

Form

Ascites fluid, 0.03% sodium azide.

Specificity:

Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ota, N. et al. 2001. J. Hum. Genet. 46: 267-272.