Technical Data
030340
INCENP (Inner centromere protein)
Description:
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF:mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

Applications:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Immunocytochemistry: 1:200-1:1000
Flow Cytometry: 1:200-1:400
ELISA: 1:10000

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B418
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. coli.
Purity:
Purified
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.J Cell Biol. 2009 Nov 30;187(5):637-53.
2.Genes Cells. 2011 Jun;16(6):652-69.