Technical Data
030483
NEFH (Neurofilament Heavy Polypeptide, NF-H, 200kD Neurofilament Protein, Neurofilament Triplet H Protein, KIAA0845, NFH)
Description:
Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglian cells. Neurofilaments consist of three major subunits with molecular weights of 68kD, 160kD and 200kD. This antibody will help detect the neurofilament 200kD subunit in normal and uncharacterized tissues.

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Applications:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000

ELISA: 1:10000 proposed

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B561
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified recombinant fragment of human NEFH (AA: 968-1020) expressed in E. coli.
Purity:
Purified
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.J Neurol Sci. 2011 May 15;304(1-2):117-21. 2.Neurochem Res. 2011 Dec;36(12):2287-91.