Technical Data
030484
NEFL
Description:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Applications:
Western Bloting: 1:500-1:2000.
Immunohistochemistry: 1:200-1:1000.
Immunofluorescence: 1:200-1:1000.
Flow cytometry: 1:200-1:400.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B562
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified recombinant fragment of human NEFL expressed in E. coli.
Purity:
Purified
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. BMB Rep. 2008 Dec 31;41(12):868-74.
2. J Hum Genet. 2009 Feb;54(2):94-7.