Technical Data
030485
NEFL
Description:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:200-1:400
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Immunocytochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B563Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant protein fragment of human NEFL expressed in E. coli.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 0.5% protein stabilizer.
Specificity:
Recognizes human NEFL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. BMB Rep. 2008 Dec 31;41(12):868-74.
2. J Hum Genet. 2009 Feb;54(2):94-7.