Technical Data
030685
Thioredoxin (TRX)
Description:
Thioredoxin (TRX) is a small ubiquitous protein (MW12kD) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation. The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.

Applications:
Western Bloting: 1:500-1:2000.
ELISA: Suggested dilution 1:10000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B763
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fusion protein with Thioredoxin (TRX) tag.
Purity:
Ascites
Form
Ascites fluid, 0.03% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985).
2. Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988).
3. Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).