Technical Data
030734
AC133 (Prominin-like protein 1, Antigen AC133, CD133 antigen, PROM1, PROML1)
Description:
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100~500
Immunofluorescence: 1:10~50
Immunohistochemistry

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG132AT1672Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
0.25mg/ml
Immunogen:
KLH conjugated synthetic peptide selected from the N-terminal region of AC133.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lugli A, et al., Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer. Br J Cancer, 2010 Jul 27. PMID 20606680. 2. Annabi B, et al., Mol Members of the low-density lipoprotein receptor-related proteins provide a differential molecular signature between parental and CD133+ DAOY medulloblastoma cells. Carcinog, 2010 Jul. PMID 20564348. 3. Wang YK, et al., Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells. Carcinogenesis, 2010 Aug. PMID 20530554. 4. Hibi K, et al., Demethylation of the CD133 gene is frequently detected in early gastric carcinoma. Anticancer Res, 2010 Apr. PMID 20530428. 5. Elsaba TM, et al., The stem cell marker CD133 associates with enhanced colony formation and cell motility in colorectal cancer. PLoS One, 2010 May 19. PMID 20502714.