Technical Data
030775
ANPEP/CD13 (ANPEP, APN, CD13, PEPN, Aminopeptidase N, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD_antigen=CD13)
Description:
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Applications:
Suitable for use in Western Blot, ELISA.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100~250

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a505CT12.1.2Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified His-tagged ANPEP protein (fragment).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Curnis, F., et al. J. Biol. Chem. 285(12):9114-9123(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010)
Ito, S., et al. Gen Thorac Cardiovasc Surg 57(11):591-598(2009)
Ju, S., et al. Cell Cycle 8(16):2578-2585(2009)