This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Suitable for use in .
Western Blot: 1:200~1000
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|Purified His-tagged APG7 protein.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744.|
Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Zhao Y, et al. Nat Cell Biol, 2010 Jul. PMID 20543840.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Atg7 deficiency increases resistance of MCF-7 human breast cancer cells to photodynamic therapy. Xue LY, et al. Autophagy, 2010 Feb. PMID 20083906.
Proteasome inhibitors activate autophagy as a cytoprotective response in human prostate cancer cells. Zhu K, et al. Oncogene, 2010 Jan 21. PMID 19881538.