Technical Data
030784
APOA1, NT (APOA1, Apolipoprotein A-I, Apolipoprotein A1, Truncated apolipoprotein A-I, Apolipoprotein A-I(1-242))
Description:
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1464CT10.4.4Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-37 in the N-terminal region of human APOA1, UniProt Accession #P02647.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human APOA1. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Clemente-Postigo, M., et al. Clin. Biochem. 43 (16-17), 1300-1304 (2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) Dawar, R., et al. Am. J. Clin. Pathol. 134(2):249-255(2010) Mattei, J., et al. Nutr Metab Cardiovasc Dis (2010) In press Bencharif, K., et al. Lipids Health Dis 9, 75 (2010)