Technical Data
030805
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197)
Description:
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:50 (Ref.2)
Immunohistochemistry: 1:50-1:200

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG3,k15CT26Affinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis. Niu TK, et al. FEBS Lett, 2010 Aug 20. PMID 20638385. Regulation of amyloid precursor protein processing by the Beclin 1 complex. Jaeger PA, et al. PLoS One, 2010 Jun 15. PMID 20559548. Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors. Li Z, et al. BMC Cancer, 2010 Mar 16. PMID 20230646. Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells. Sun Y, et al. Cancer Lett, 2010 Aug 28. PMID 20207475.