Technical Data
030806
Beclin-1 (Coiled-coil Myosin-like BCL2-interacting Protein, Protein GT197, BECN1, GT197)
Description:
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100~400
Immunohistochemistry: 1:50~100

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM,k117CT2.5.1.1Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
BECN1 Mab was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Purity:
Ascites
Form
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis. Niu TK, et al. FEBS Lett, 2010 Aug 20. PMID 20638385. Regulation of amyloid precursor protein processing by the Beclin 1 complex. Jaeger PA, et al. PLoS One, 2010 Jun 15. PMID 20559548. Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors. Li Z, et al. BMC Cancer, 2010 Mar 16. PMID 20230646. Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells. Sun Y, et al. Cancer Lett, 2010 Aug 28. PMID 20207475.