Technical Data
030951
ESR1/ER (ESR1, ESR, NR3A1, Estrogen receptor, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1)
Description:
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1415CT16.3.2.1Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a portion of amino acids within aa246-273 from human ESR1 protein, conjugated to KLH (NP_005294.1). Species sequence homology: bovine, mouse, porcine, rat
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognized human ESR1 at ~31kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Geradts, J., et al. Cancer Invest. 28(9):969-977(2010)
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Corbo, R.M., et al. J. Gerontol. A Biol. Sci. Med. Sci. (2010) In press
Kim, S., et al. Fertil. Steril. (2010) In press