Technical Data
031079
LEF1 (LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha)
Description:
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Applications:
Suitable for use in Western Blot, ELISA.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:500~8000

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1661CT8.3.5Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
LEF1 Mab is generated from mice immunized with a KLH conjugated synthetic peptide selected from human LEF1.
Purity:
Ascites
Form
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gutierrez, A. Jr., et al. Blood 116(16):2975-2983(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Chen, Q.Y., et al. J. Immunol. 184(9):5047-5054(2010)
Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)