Technical Data
Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Suitable for use in Western Blot, Immunofluorescence, ELISA.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100~200
Immunofluorescence: 1:10~50

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanMouse
As reported
Nephrin Mab was raised using purified His-tagged recombinant human Nephrin.
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010) Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010) Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010) Aya, K., et al. Kidney Int. 57(2):401-404(2000)