Technical Data
031175
Osteonectin, CT (ON, Basement-membrane Protein 40, BM-40, Secreted Protein Acidic and Rich in Cysteine, SPARC)
Description:
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).

Applications:
Suitable for use in Western Blot, ELISA.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100~800

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1664CT7.3.1Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
SPARC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
Purity:
Ascites
Form
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Howard, C., et al. Histol. Histopathol. 25(9):1163-1169(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)
Liang, J.F., et al. J. Exp. Clin. Cancer Res. 29, 71 (2010)