Technical Data
031303
TOP2A (TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme)
Description:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Applications:
Suitable for use in Western Blot, ELISA.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:500~8000

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1548CT6.1.1Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified His-tagged TOP2A protein (fragment).
Purity:
Ascites
Form
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Vranic, S., et al. Hum. Pathol. 41(11):1617-1623(2010)
Chen, H., et al. Am. J. Surg. Pathol. 34(9):1250-1257(2010)
Ye, J., et al. Cell 142(2):230-242(2010)
Rossi, E., et al. Histopathology 57(1):81-89(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)