Technical Data
031347
14-3-3 eta (14-3-3 Protein eta, Protein AS1, YWHAH, YWHA1)
Description:
The 14-3-3 family of proteins were originally discovered as prominent protein spots on 2 dimensional gels. They are a family of 28-33kD proteins which are often major proteins of the cytoplasm (1). They function as binding partners for phosphoserine and phosphothreonine sites in other proteins, though they have other binding partners which are not dependent on phosphorylation. These binding interactions are important in the regulation of molecules such as the signaling kinases in the MAP kinase pathway, c-Raf and b-Raf, the proapoptotic molecules Bad and Bax, and the cell cycle regulator Cdc25. There are 7 mammalian 14-3-3 proteins and they are normally expressed as homo or in some cases heterodimers. 14-3-3 or 14-3-3 eta is widely expressed and concentrated in the nervous system. The HGNC name for 14-3-3 is YWHAH, and an alternate name is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 1, due to a role for this protein in activation of tyrosine and tryptophan hydroxylases. One result of this is that the HGNC name is YWHAH. The 14-3-3 protein accumulates in the CSF of patients suffering form Creutzfeld Jacob Disease, and can be used for the diagnosis of this disease (2). Furthermore, this protein binds -synuclein in the Lewy bodies of Parkinson's disease and has been linked to early-onset schizopherenia (3,4). The HGNC name for this protein is YWHAH.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500-1:1000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113B811Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Full length recombinant 14-3-3 eta expressed in and purified from E. coli.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid.
Specificity:
Recognizes 14-3-3 eta from human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morrison, DK. The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development. Trends Cell Biol. 19:16-23 (2009).

2. Hsich G, Kenney K, Gibbs CJ, Lee KH and Harrington MG. The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. N Engl J Med. 335:924-30 (1996).

3: Ubl A, Berg D, Holzmann C, Krüger R, Berger K, Arzberger T, Bornemann A, Riess O. 14-3-3 protein is a component of Lewy bodies in Parkinson's disease-mutation analysis and association studies of 14-3-3 eta. Brain Res Mol Brain Res. 108(1-2):33-9 (2002).

4: Middleton FA, Peng L, Lewis DA, Levitt P, Mirnics K. Altered expression of 14-3-3 genes in the prefrontal cortex of subjects with schizophrenia. Neuropsychopharmacology 30(5):974-83 (2005).