Technical Data
Internexin, alpha (alpha-Internexin, alpha-Inx, INA, Inexa, 66kD Neurofilament Protein, Neurofilament-66, NF66, NF-66, Neurofilament 5, NEF5)
a-Internexin is a Class IV intermediate filament subunit. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H, and a-internexin is the major neurofilament protein of developing neurons. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The HGNC name for this protein is INA.

Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry (ABC): 1:5000
Immunofluorescence: 1:500
Western Blot: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceRatMouse
Not determined
Purified recombinant rat a-internexin expressed in and purified from E. coli.
Supplied as a liquid.
Recognizes human a-Internexin and with this protein in all mammalian species tested to date.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, Shaw G. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 2002.