Technical Data
031421
Peripherin (Prph, Prph1)
Description:
Peripherin is a ~57kD intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name (1). The HGNC name for this protein is PRPH. Subsequently peripherin was found in some sensory and other neurons of the central nervous system and also in rat pheochromacytoma PC12 cells (2,3). Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration. The HGNC name for this protein is PRPH.

Applications:
Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: For cells in tissue culture or in tissue sections, try this antibody at about between about 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methods.
Western Blot: 1:20,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Recombinant full length peripherin purified from E. coli.
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
Recognizes rat Peripherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Portier MM, de Néchaud B, Gros F. Peripherin, a new member of the intermediate filament protein family. Dev Neurosci. 6:335-44 (1984).

2. Troy CM, Brown K, Greene LA, Shelanski ML. Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo. Neuroscience. 36:217-37 (1990).

3. Aletta JM, Angeletti R, Liem RK, Purcell C, Shelanski ML, Greene LA. Relationship between the nerve growth factor-regulated clone 73 gene product and the 58-kilodalton neuronal intermediate filament protein (peripherin).J Neurochem. 51:1317-20 (1988).

4. Errante LD, Wiche G and Shaw G. Distribution of plectin, an intermediate filament-associated protein, in the adult rat central nervous system J. Neurosci. Res. 37:515-528 (1994).