Technical Data
031451
5NT1A, NT (NT5C1A, Cytosolic 5'-nucleotidase 1A, Cytosolic 5'-nucleotidase IA)
Description:
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates. Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 91-119 in the N-terminal region of human 5NT1A, UniProt Accession #Q9BX13 Cellular Location: Cytoplasm.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human 5NT1A. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009). 2. Lechward, K., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(4):280-284(2009). 3. Fyrberg, A., et al. Nucleosides Nucleotides Nucleic Acids 25(9-11), 1201-1204 (2006).