Technical Data
031502
ABL, phosphorylated (Y412) (p150, Proto-oncogene c-Abl, Abelson murine leukemia viral oncogene homolog 1, Tyrosine-protein kinase ABL1, JTK7, ABL, ABL1)
Description:
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.

Applications:
Suitable for use in Dot Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Phospho-ABL-Y412 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y412 of human ABL.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fabbro, D., et al. Biochim. Biophys. Acta 1804(3):454-462(2010)
Kim, M.J., et al. Cancer Genet. Cytogenet. 196(2):184-188(2010)
Lewandowski, K., et al. Pol. Arch. Med. Wewn. 119(12):789-794(2009)
Li, Y.N., et al. Zhonghua Nei Ke Za Zhi 48(6):481-484(2009)
Wissing, J., et al. Mol. Cell Proteomics 6(3):537-547(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)