Technical Data
031538
ACK1, phosphorylated (Y826) (TNK2, ACK1, Activated CDC42 kinase 1, Tyrosine kinase non-receptor protein 2)
Description:
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Applications:
Suitable for use in Dot Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Dot Blot 1:500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Phospho-ACK1-Y826 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 814~844 amino acids surrounding Y826 of human ACK1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Guo A, et al. (2008) A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203
Huang PH, et al. (2007) Proc Natl Acad Sci U S A 104, 12867-72
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5
Goss VL, et al. (2006) Blood 107, 4888-97
Wolf-Yadlin A, et al. (2006) Mol Syst Biol 2, 54
Rush J, et al. (2005) Nat Biotechnol 23, 94-101